In the production of large flanges, there are many factors that affect the performance of large flanges. Below, we will discuss several common factors, First, the annealing temperature The annealing temperature is the temperature parameter at which primers and templates bind. It is the temperature at which 50% of primers and complementary sequences exhibit double stranded DNA molecules. It is a significant factor affecting PCR specificity. In an ideal state, the annealing temperature is low enough to anneal primers with the same target sequence, while also high enough to reduce non-specific binding. A reasonable annealing temperature ranges from 55 ℃ to 70 ℃. The annealing temperature is generally set to be 5 ℃ lower than the Tm of primers .) Whether the specified temperature has been reached. Large flange treatment generally adopts solid solution heat treatment, which is commonly referred to as "; Annealing;, The temperature range is 1040~1120 ℃ (Japanese standard). You can also observe through the observation hole of the annealing furnace. The large flange fittings in the annealing area should be in an incandescent state, but there should be no softening or sagging

Next is the sealing of the furnace body (the shell is welded with steel plates and profiles, and the trolley is welded with profiles and steel plates. The trolley reduces heat radiation and convection losses through soft contact with the furnace lining and sand sealing mechanism, and the sealing of the furnace body.). The bright annealing furnace should be closed and isolated from external air; When hydrogen gas is used as the protective gas, only one exhaust port is open (used to ignite the discharged hydrogen gas). The method of inspection can be to apply soap and water to the gaps of various joints in the annealing furnace to see if there is any gas leakage; The areas where gas is prone to escape are the inlet and outlet of the annealing furnace, and the sealing rings in these areas are particularly prone to wear and tear, requiring frequent inspection and replacement

Next is the pressure of the protective gas. In order to prevent slight leakage, the positive pressure of the protective gas in the furnace should be maintained. If it is hydrogen protective gas, it is generally required to be above 20kBar. Annealing atmosphere: Pure hydrogen is generally used as the annealing atmosphere, with a purity of above. If the other part of the atmosphere is an inert gas, the purity can also be lower, but it cannot contain too much oxygen or water vapor